Regulatory
Part:BBa_J64800:Design
Designed by: laura lavery Group: Voigt Lab (2007-03-24)
RHLR/RHLI Inducible & LasR/LasI repressible Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a 17bp spacer between the -35 and -10 regions and the binding site for LasR/LasI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the LasR/LasI binding site are the same.